N-succinyl chitosan/chondroitin sulfate oxide hydrogel /CS/AMPS/PEGDA cross-linked network porous hydrogel

NCS/OCS composite hydrogel was prepared by combining N-succinyl chitosan (NCS) with oxychondroitin sulfate (OCS). The effects of different mass ratios of OCS and NCS on the gelation time, compression strength, equilibrium swelling and in vitro degradation of hydrogels were investigated. The results showed that when m(NCS): M (OCS) was 7:3, the compound hydrogel could meet the clinical requirements. At this time, the gel time of the compound hydrogel at 37℃ was about 16 min, the compression strength was (5.82±0.5)kPa, and the residual mass fraction of the compound hydrogel was about 40% after 30 days. The gel strength and degradation performance of injectable hydrogels were significantly improved by the combination of oxychondroitin sulfate and N-succinyl chitosan, which is expected to be used in cartilage tissue engineering scaffolds.

Porous hydrogels with CS/AMPS/PEGDA crosslinked network were prepared from chitosan (CS), 2-acrylamide-2-methylpropanesulfonic acid (AMPS) and polyethylene glycol diacrylate (PEGDA) by photopolymerization and freeze drying. The chemical structure of the hydrogel was analyzed by INFRARED spectroscopy (FTIR), the double bond conversion of the monomer was monitored by REAL-TIME INFRARED spectroscopy (RTIR), the morphology of the hydrogel was observed by scanning electron microscopy (SEM), and the swelling property and thermal stability of the hydrogel were tested. The results show that when the mass ratio of CS/AMPS is 1/2, the hydrogels have regular porous structure, high porosity and high equilibrium swelling ratio.